Composite
Part:BBa_K1433002:Design
Designed by: Hanqing Liu Group: iGEM14_ZJU-China (2014-09-28)
attL-J23110-attR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 51
Illegal NheI site found at 74 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 24
Illegal BsaI.rc site found at 107
Design Notes
No
Source
this parts are construct from BBa_J23110 by adding attL/attR sites using PCR
References
- Bonnet J, Subsoontorn P, Endy D. Rewritable digital data storage in live cells via engineered control of recombination directionality[J]. Proceedings of the National Academy of Sciences, 2012, 109(23): 8884-8889.
- Siuti P, Yazbek J, Lu T K. Synthetic circuits integrating logic and memory in living cells[J]. Nature biotechnology, 2013, 31(5): 448-452.